Journal:

Article Title: Effect of notoginsenoside R1 on hepatic microcirculation disturbance induced by gut ischemia and reperfusion

doi: 10.3748/wjg.14.29

Figure Lengend Snippet: Effect of R1 on the expression of E-selectin in hepatic vessels (A) and CD18 and CD11b in neutrophils (B) of mice subjected to SMA I/R. The results are presented as mean ± SE from 6 animals. aP < 0.05 vs control, cP < 0.05 vs I/R.

Article Snippet: Other reagents used in experiments were as follows: rhodamine 6G (purity > 99.0%, Lot No.2350994, Fluka Co., Switzerland), FITC-rat anti-mouse CD18 monocolonal antibody (Lot No.553293, BD Biosciences PharMingen, USA), FITC-rat anti-mouse CD11b monoclonal antibody (Lot No.557396, BD Biosciences PharMingen, USA), goat polyclonal antibody against mouse E-selectin (M-20) (sc-6939, Santa Cruz Biotechnology, Inc. USA), goat polyclonal antibody against mouse ICAM-1 (M-19) (sc-1511, Santa Cruz Biotechnology, Inc. USA), rhodamine conjugated rabbit anti-goat lgG-R (Lot No.B1006, Santa Cruz Biotechnology, Inc. USA), Hoechst33342 (Lot No.6538, Santa Cruz Biotechnology, Inc. USA), mouse MCP-1 flex set (Lot No.558342, BD Biosciences, USA), mouse TNF flex set (Lot No.558299, BD Biosciences, USA), mouse IL-6 flex set (Lot No.558301, BD Biosciences, USA).

Techniques: Expressing

Journal: Acta biomaterialia

Article Title: In vivo short-term and long-term host reaction to starch-based scaffolds.

doi: 10.1016/j.actbio.2010.06.020

Figure Lengend Snippet: Fig. 2. Micrographs of the sections of the explanted FB and WS produced starch-based scaffolds after 1 (A–D) and 2 (E–H) weeks of IM implantation in rats. Tissue was immunohistochemically labelled for CD18 and CD3. represents the SPCL scaffold fibres, arrows mark CD18-positive cells and dashed arrows mark CD3-positive cells.

Article Snippet: The histological analysis of crosssection samples was performed after haematoxylin and eosin (HE), and Masson Goldner Trichrome (MGT) staining and immunohistochemistry using a monoclonal mouse anti-human CD3 antibody (Dako, Denmark) with cross-reactivity for rat T lymphocytes, a monoclonal mouse anti-rat CD18 antibody (Serotec, UK) for the integrin b2 chain of recruited leukocytes, and a monoclonal mouse anti-human phosphoinositide 3-Kinase (Pi3K) antibody (BD, Belgium) with cross-reactivity for rat activated and proliferating lymphocytes, following standard protocols.

Techniques: Produced, Starch

Journal: Acta biomaterialia

Article Title: In vivo short-term and long-term host reaction to starch-based scaffolds.

doi: 10.1016/j.actbio.2010.06.020

Figure Lengend Snippet: Fig. 5. Micrographs of the sections of the explanted FB and WS produced starch-based scaffolds after 1 (A–D) and 2 (E–H) weeks of SC implantation in rats. Tissue was immunohistochemically labelled for CD18 and CD3. represents the SPCL scaffold fibres, arrows mark CD18-positive cells and dashed arrows mark CD3-positive cells.

Article Snippet: The histological analysis of crosssection samples was performed after haematoxylin and eosin (HE), and Masson Goldner Trichrome (MGT) staining and immunohistochemistry using a monoclonal mouse anti-human CD3 antibody (Dako, Denmark) with cross-reactivity for rat T lymphocytes, a monoclonal mouse anti-rat CD18 antibody (Serotec, UK) for the integrin b2 chain of recruited leukocytes, and a monoclonal mouse anti-human phosphoinositide 3-Kinase (Pi3K) antibody (BD, Belgium) with cross-reactivity for rat activated and proliferating lymphocytes, following standard protocols.

Techniques: Produced, Starch

Journal: Cell & Bioscience

Article Title: Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue

doi: 10.1186/s13578-025-01401-1

Figure Lengend Snippet: Immunofluorescence and western blotting results showing the expression of relevant cytokines in the CIBT of 2VO + EMS rats. A Representative triple immunofluorescence staining images showing ANGPT2 + , Tie2 + , and CD11b + cells in the CIBT in each group (white arrows indicate TEMs). Bar = 50 µm. B Counts of ANGPT2 + cells in each group (n = 3, one-way ANOVA and Šídák's multiple comparisons test). C Counts of TEMs (Tie2 + and CD11b + ) in each group (n = 9, one-way ANOVA and Šídák's multiple comparisons test). D Representative western blot showing the relative expression of TRPS1, ANGPT2, Tie2, CD11b, VEGFA, IGF1, and CD31 in the CIBT adjacent to the TM tissue in each group (normalized to β-actin expression). E Densitometric analyses of the relative expression of TRPS1, ANGPT2, Tie2, CD11b, VEGFA, IGF1, and CD31 (n = 3, one-way ANOVA and Šídák's multiple comparisons test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). The error bars represent the ± SDs. CIBT: chronically ischaemic brain tissue; EMS: encephalomyosynangiosis; TEMs: Tie2-expressing monocytes/macrophages; 2VO: 2-vessel occlusion

Article Snippet: After being washed with PBST, the slices were incubated with 488-conjugated goat anti-rabbit (1:2000, ab150077, Abcam, UK), 594-conjugated goat anti-mouse (1:2000, ab150116, Abcam, UK), and 647-conjugated donkey anti-goat (1:2000, ab150135, Abcam, UK) secondary antibodies at room temperature in a dark room for 2 h. The 647-conjugated CD11b antibody (0.5 μg, CL647-65229, proteintech, China) was used to directly label CD11b.

Techniques: Immunofluorescence, Western Blot, Expressing, Staining

Journal: Cell & Bioscience

Article Title: Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue

doi: 10.1186/s13578-025-01401-1

Figure Lengend Snippet: Immunofluorescence results showing the expression of pro-angiogenesis factors in the CIBT of 2VO + EMS rats. A Representative triple immunofluorescence staining images showing Tie2 + , CD11b + , and VEGFA + cells and in the CIBT in each group (white arrows indicate TEMs). Bar = 50 µm. B Counts of VEGF + cells in each group (n = 3, one-way ANOVA and Šídák's multiple comparisons test). C Representative triple immunofluorescence staining images showing Tie2 + , CD11b + , and IGF1 + cells in the CIBT in each group (white arrows indicate TEMs). Bar = 50 µm. D Counts of IGF1 + cells in each group (n = 3, one-way ANOVA and Šídák's multiple comparisons test). CIBT: chronically ischaemic brain tissue; EMS: encephalomyosynangiosis; TEMs: Tie2-expressing monocytes/macrophages; 2VO: 2-vessel occlusion

Article Snippet: After being washed with PBST, the slices were incubated with 488-conjugated goat anti-rabbit (1:2000, ab150077, Abcam, UK), 594-conjugated goat anti-mouse (1:2000, ab150116, Abcam, UK), and 647-conjugated donkey anti-goat (1:2000, ab150135, Abcam, UK) secondary antibodies at room temperature in a dark room for 2 h. The 647-conjugated CD11b antibody (0.5 μg, CL647-65229, proteintech, China) was used to directly label CD11b.

Techniques: Immunofluorescence, Expressing, Staining

Journal: PLoS ONE

Article Title: Label-Free Proteomics Reveals Decreased Expression of CD18 and AKNA in Peripheral CD4 + T Cells from Patients with Vogt-Koyanagi-Harada Syndrome

doi: 10.1371/journal.pone.0014616

Figure Lengend Snippet: The differently expressed proteins identified based on single peptides in CD4 + T cells between active VKH patients and normal individuals.

Article Snippet: Membranes were incubated with antibodies at dilutions of 1∶1000 for both anti-human CD18 mAb (R&D systems, Minneapolis, MN) and anti-human AKNA mAb (Genway Biotech, San Diego, CA).

Techniques: Ubiquitin Proteomics

Journal: PLoS ONE

Article Title: Label-Free Proteomics Reveals Decreased Expression of CD18 and AKNA in Peripheral CD4 + T Cells from Patients with Vogt-Koyanagi-Harada Syndrome

doi: 10.1371/journal.pone.0014616

Figure Lengend Snippet: Antibodies were used at a dilution of 1∶1000 for the anti-human CD18 monoclonal antibody and anti-human AKNA monoclonal antibody. Proteins were detected using the Phototope-HRP Western blot detection system(A). The immunoreactive band intensities were quantitated and were presented as intensity volumes (vol%). The results showed that both CD18 and AKNA were significantly down-regulated in VKH patients as compared to normal controls (B). VKH: VKH patients, NC: normal controls.

Article Snippet: Membranes were incubated with antibodies at dilutions of 1∶1000 for both anti-human CD18 mAb (R&D systems, Minneapolis, MN) and anti-human AKNA mAb (Genway Biotech, San Diego, CA).

Techniques: Western Blot

Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: For DKK1-, SPARC-, TIMP2-, COL1, integrin β2, or integrin α3 PLA, purified rabbit monoclonal anti-DKK1 (1:200, Cell Signaling Technology, Cat# 48367S), rabbit polyclonal anti-SPARC (1:200, Cell Signaling Technology, Cat# 5420S), mouse monoclonal anti-TIMP2 (1:200, R&D Systems, Cat# MAB971), mouse monoclonal anti-COL1 (1:200, Cell Signaling Technology, Cat# 66948S), goat polyclonal anti-integrin β1 (1:200, R&D Systems, Cat# AF1778), goat polyclonal anti-integrin β2 (1:200, R&D Systems, Cat# AF1730), and mouse monoclonal anti-integrin α3 (1:200, R&D Systems, Cat# MAB1345) were used.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western